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primary antibodies targeting human fasn, gli2, pdgfra, vhl or β-actin  (Proteintech)


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    Proteintech primary antibodies targeting human fasn, gli2, pdgfra, vhl or β-actin
    Primary Antibodies Targeting Human Fasn, Gli2, Pdgfra, Vhl Or β Actin, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fasn/anti+gli2/pm40348840-87-6-19
    Average 90 stars, based on 1 article reviews
    primary antibodies targeting human fasn, gli2, pdgfra, vhl or β-actin - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2-AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature.
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2- AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    Incubation:

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2-AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature.
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2- AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    SDS-Gel:

    Article Title: Impact of FHIT loss on the translation of cancer-associated mRNAs.
    Article Snippet: Whole cell extracts were prepared as above, but nuclei were disrupted with three 10 s pulses (5 min rest on ice between each pulse) using a Fisher Scientific 60 sonic dismembrator fitted with a microtip on setting 3 prior to the final centrifugation step. .. SDS Laemmli loading dye was added to cytoplasmic or whole cell extracts harvested as above and samples were heated at 95 °C for 5 min. Proteins were separated by SDS gel electrophoresis, transferred to Immobilon FL (EMD millipore) PVDF membranes, and immunoblotted with antisera against human Fhit (1:1000, [22], human eEEF2 (1:1000, One World Labs), human TP53I3 (1:1000, One World Labs), human IFIT1 (1:600, Protein-Tech), human Vinculin (1: 5000, Abcam), human ADAM9 (1:1000, GeneTex) and human FASN (1:1000, Proteintech). .. IR-680 or IR-800 conjugated goat anti-rabbit or anti-mouse secondary antibodies were used at 1:5000 dilutions and blots were scanned and bands analyzed using a Licor Odyssey Imager.

    Article Title: Impact of FHIT loss on the translation of cancer-associated mRNAs
    Article Snippet: Whole cell extracts were prepared as above, but nuclei were disrupted with three 10 s pulses (5 min rest on ice between each pulse) using a Fisher Scientific 60 sonic dismembrator fitted with a microtip on setting 3 prior to the final centrifugation step. .. SDS Laemmli loading dye was added to cytoplasmic or whole cell extracts harvested as above and samples were heated at 95 °C for 5 min. Proteins were separated by SDS gel electrophoresis, transferred to Immobilon FL (EMD millipore) PVDF membranes, and immunoblotted with antisera against human Fhit (1:1000, [ ], human eEEF2 (1:1000, One World Labs), human TP53I3 (1:1000, One World Labs), human IFIT1 (1:600, Protein-Tech), human Vinculin (1: 5000, Abcam), human ADAM9 (1:1000, GeneTex) and human FASN (1:1000, Proteintech). .. IR-680 or IR-800 conjugated goat anti-rabbit or anti-mouse secondary antibodies were used at 1:5000 dilutions and blots were scanned and bands analyzed using a Licor Odyssey Imager.

    Electrophoresis:

    Article Title: Impact of FHIT loss on the translation of cancer-associated mRNAs.
    Article Snippet: Whole cell extracts were prepared as above, but nuclei were disrupted with three 10 s pulses (5 min rest on ice between each pulse) using a Fisher Scientific 60 sonic dismembrator fitted with a microtip on setting 3 prior to the final centrifugation step. .. SDS Laemmli loading dye was added to cytoplasmic or whole cell extracts harvested as above and samples were heated at 95 °C for 5 min. Proteins were separated by SDS gel electrophoresis, transferred to Immobilon FL (EMD millipore) PVDF membranes, and immunoblotted with antisera against human Fhit (1:1000, [22], human eEEF2 (1:1000, One World Labs), human TP53I3 (1:1000, One World Labs), human IFIT1 (1:600, Protein-Tech), human Vinculin (1: 5000, Abcam), human ADAM9 (1:1000, GeneTex) and human FASN (1:1000, Proteintech). .. IR-680 or IR-800 conjugated goat anti-rabbit or anti-mouse secondary antibodies were used at 1:5000 dilutions and blots were scanned and bands analyzed using a Licor Odyssey Imager.

    Article Title: Impact of FHIT loss on the translation of cancer-associated mRNAs
    Article Snippet: Whole cell extracts were prepared as above, but nuclei were disrupted with three 10 s pulses (5 min rest on ice between each pulse) using a Fisher Scientific 60 sonic dismembrator fitted with a microtip on setting 3 prior to the final centrifugation step. .. SDS Laemmli loading dye was added to cytoplasmic or whole cell extracts harvested as above and samples were heated at 95 °C for 5 min. Proteins were separated by SDS gel electrophoresis, transferred to Immobilon FL (EMD millipore) PVDF membranes, and immunoblotted with antisera against human Fhit (1:1000, [ ], human eEEF2 (1:1000, One World Labs), human TP53I3 (1:1000, One World Labs), human IFIT1 (1:600, Protein-Tech), human Vinculin (1: 5000, Abcam), human ADAM9 (1:1000, GeneTex) and human FASN (1:1000, Proteintech). .. IR-680 or IR-800 conjugated goat anti-rabbit or anti-mouse secondary antibodies were used at 1:5000 dilutions and blots were scanned and bands analyzed using a Licor Odyssey Imager.



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    a Schematic diagram of circular RNA-mediated inverse prime editors (ciPEs). b Schematic diagrams of the structure of ciPE editors. CMV, the CMV promoter of cytomegalovirus; <t>U6,</t> the polymerase III promoter of U6; NLS, bipartite nuclear localization signal; M-MLV RTΔRNase H, deletion variant of M-MLV RT with no RNase H domain; 5′ RL, 5′ ribozyme and ligation sequences; 3′ RL, 3′ ribozyme and ligation sequences; RTT, reverse transcriptase template; PBS, primer binding site. c Comparison of inverse prime editing frequencies between split iPE2 and ciPE2 at four target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in c were obtained from three biological replicates ( n = 3). d Comparison of inverse prime editing efficiencies between ciPE2–5 and split iPEmax2–5 <t>using</t> <t>epegRNA</t> at six target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in d were obtained from four biological replicates ( n = 4). circRNA, circular RNA; Ins, insertion; Del, deletion. InDels, byproducts of random insertions and deletions. P values were obtained from two-tailed Student’s t -test: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.
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    a Schematic diagram of circular RNA-mediated inverse prime editors (ciPEs). b Schematic diagrams of the structure of ciPE editors. CMV, the CMV promoter of cytomegalovirus; <t>U6,</t> the polymerase III promoter of U6; NLS, bipartite nuclear localization signal; M-MLV RTΔRNase H, deletion variant of M-MLV RT with no RNase H domain; 5′ RL, 5′ ribozyme and ligation sequences; 3′ RL, 3′ ribozyme and ligation sequences; RTT, reverse transcriptase template; PBS, primer binding site. c Comparison of inverse prime editing frequencies between split iPE2 and ciPE2 at four target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in c were obtained from three biological replicates ( n = 3). d Comparison of inverse prime editing efficiencies between ciPE2–5 and split iPEmax2–5 <t>using</t> <t>epegRNA</t> at six target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in d were obtained from four biological replicates ( n = 4). circRNA, circular RNA; Ins, insertion; Del, deletion. InDels, byproducts of random insertions and deletions. P values were obtained from two-tailed Student’s t -test: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.
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    Image Search Results


    a Schematic diagram of circular RNA-mediated inverse prime editors (ciPEs). b Schematic diagrams of the structure of ciPE editors. CMV, the CMV promoter of cytomegalovirus; U6, the polymerase III promoter of U6; NLS, bipartite nuclear localization signal; M-MLV RTΔRNase H, deletion variant of M-MLV RT with no RNase H domain; 5′ RL, 5′ ribozyme and ligation sequences; 3′ RL, 3′ ribozyme and ligation sequences; RTT, reverse transcriptase template; PBS, primer binding site. c Comparison of inverse prime editing frequencies between split iPE2 and ciPE2 at four target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in c were obtained from three biological replicates ( n = 3). d Comparison of inverse prime editing efficiencies between ciPE2–5 and split iPEmax2–5 using epegRNA at six target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in d were obtained from four biological replicates ( n = 4). circRNA, circular RNA; Ins, insertion; Del, deletion. InDels, byproducts of random insertions and deletions. P values were obtained from two-tailed Student’s t -test: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Circular RNA-mediated inverse prime editing in human cells

    doi: 10.1038/s41467-025-59120-7

    Figure Lengend Snippet: a Schematic diagram of circular RNA-mediated inverse prime editors (ciPEs). b Schematic diagrams of the structure of ciPE editors. CMV, the CMV promoter of cytomegalovirus; U6, the polymerase III promoter of U6; NLS, bipartite nuclear localization signal; M-MLV RTΔRNase H, deletion variant of M-MLV RT with no RNase H domain; 5′ RL, 5′ ribozyme and ligation sequences; 3′ RL, 3′ ribozyme and ligation sequences; RTT, reverse transcriptase template; PBS, primer binding site. c Comparison of inverse prime editing frequencies between split iPE2 and ciPE2 at four target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in c were obtained from three biological replicates ( n = 3). d Comparison of inverse prime editing efficiencies between ciPE2–5 and split iPEmax2–5 using epegRNA at six target sites in HEK293T cells. Frequencies (mean ± s.e.m.) in d were obtained from four biological replicates ( n = 4). circRNA, circular RNA; Ins, insertion; Del, deletion. InDels, byproducts of random insertions and deletions. P values were obtained from two-tailed Student’s t -test: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.

    Article Snippet: The sgRNA, nicking sgRNA, pegRNA, and epegRNA vectors driven by the human U6 promoter were constructed by annealing oligonucleotides and inserting them into the pOsU3 backbone (Addgene #170132) using the Golden Gate assembly method.

    Techniques: Variant Assay, Ligation, Reverse Transcription, Binding Assay, Comparison, Two Tailed Test

    Validation of Gemcitabine resistance in BC cell lines. A CCK-8 assay was performed to detect cell viability treated with different concentrations of gemcitabine in T24, UMUC3, T24-Gem and UMUC3-Gem cells. B The protein levels of FASN, GLI2, PDGFRA and VHL in T24, T24-Gem, UMUC3, and UMUC3-Gem cells were validated by western blotting. C , D The efficiency of FASN silencing in T24-Gem and UMUC3-Gem cells was detected by qRT-PCR and western blotting. E Cell viability abilities of T24-Gem and UMUC3-Gem cells treated with different concentrations of Gemcitabine at 72 h after transfection were evaluated by CCK-8 assays. Data are presented as mean ± Standard Error of Mean (SEM) from three independent replicates. * P < 0.05, ** P < 0.01 (Student’s t test)

    Journal: Discover Oncology

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature

    doi: 10.1007/s12672-025-02581-5

    Figure Lengend Snippet: Validation of Gemcitabine resistance in BC cell lines. A CCK-8 assay was performed to detect cell viability treated with different concentrations of gemcitabine in T24, UMUC3, T24-Gem and UMUC3-Gem cells. B The protein levels of FASN, GLI2, PDGFRA and VHL in T24, T24-Gem, UMUC3, and UMUC3-Gem cells were validated by western blotting. C , D The efficiency of FASN silencing in T24-Gem and UMUC3-Gem cells was detected by qRT-PCR and western blotting. E Cell viability abilities of T24-Gem and UMUC3-Gem cells treated with different concentrations of Gemcitabine at 72 h after transfection were evaluated by CCK-8 assays. Data are presented as mean ± Standard Error of Mean (SEM) from three independent replicates. * P < 0.05, ** P < 0.01 (Student’s t test)

    Article Snippet: After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2-AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China).

    Techniques: Biomarker Discovery, CCK-8 Assay, Western Blot, Quantitative RT-PCR, Transfection

    Validation of Cisplatin resistance in BC cell lines. A CCK-8 assay was performed to detect cell viability treated with different concentrations of Cisplatin in T24, UMUC3, T24-CDDP and UMUC3-CDDP cells. B The protein levels of FASN, GLI2, PDGFRA and VHL in T24, T24-CDDP, UMUC3, and UMUC3-CDDP cells were validated by western blotting. C , D Cell viability abilities of T24-Gem and UMUC3-Gem cells treated with different concentrations of Cisplatin at 72 h after transfection were evaluated by CCK-8 assays. Data are presented as mean ± Standard Error of Mean (SEM) from three independent replicates

    Journal: Discover Oncology

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature

    doi: 10.1007/s12672-025-02581-5

    Figure Lengend Snippet: Validation of Cisplatin resistance in BC cell lines. A CCK-8 assay was performed to detect cell viability treated with different concentrations of Cisplatin in T24, UMUC3, T24-CDDP and UMUC3-CDDP cells. B The protein levels of FASN, GLI2, PDGFRA and VHL in T24, T24-CDDP, UMUC3, and UMUC3-CDDP cells were validated by western blotting. C , D Cell viability abilities of T24-Gem and UMUC3-Gem cells treated with different concentrations of Cisplatin at 72 h after transfection were evaluated by CCK-8 assays. Data are presented as mean ± Standard Error of Mean (SEM) from three independent replicates

    Article Snippet: After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2-AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China).

    Techniques: Biomarker Discovery, CCK-8 Assay, Western Blot, Transfection